Precision for Medicine offers a broad portfolio of immune monitoring assays, which now includes our proprietary and patented epigenetic technology, Epiontis ID.
Epiontis ID offers several advantages to other, more traditional assays depending on your needs—a faster, less expensive global solution for immune monitoring that delivers robust scientific data to more reliably enhance your clinical research.
Small sample volumes; no processing and no cryopreservation
Epiontis ID assays require only 2 mL whole blood to assay immune cell types. Depending on the cell type of interest, 50 to 300 µl of blood may be sufficient.
Advantages of Epiontis ID assays at a glance
- Small sample volume required (50 to 300 µl)
- No limitation in sample stability when frozen at -20°C
- Easy shipment on dry ice
- Standardized quantitative PCR technology
- Cell type assays are precise, robust, and reproducible
- Objective comparison of results between different studies
- QM, ISO 17025 accredited
Epiontis ID epigenetic cell counting—assay principle
The target genes of Epiontis ID are expressed exclusively in the cell type of interest, which is accompanied by euchromatin. Only euchromatic DNA will be reactive in the Epiontis ID format. Heterochromatic DNA from other cell types will not react with bisulfite, a chemical used in the assays. DNA from the cell type of interest is amplified in the epigenetic PCR-based assay format, resulting in a highly specific assay readout.
Cell counting principle
The following example illustrates how T cells are detected in a blood sample with Epiontis ID. In T cells, the CD3 gene region is epigenetically active (yellow); in all other cell types, this region is epigenetically inactive (blue). In each T cell, 2 epigenetically active (yellow) alleles are present and will be amplified by PCR. Non-T cells are not detected. The number of epigenetically active CD3 gene copies directly translates to the number of T cells in the sample. Parallel measurements of epigenetic reference systems, eg, housekeeping gene GAPDH or specific plasmid standards, allow for total cell number determination.
The figure illustrates the use of epigenetic markers to determine cell counts of a specific cell type.
Comparisons with flow cytometry
Epiontis ID can be valuable in validating flow cytometry measurements, as demonstrated in the figures below. The comparison of measurements of cell types between flow cytometry and the epigenetic technology of Epiontis ID demonstrates good correlation.
Epiontis ID assays represent intellectual property that is protected by patents or patent applications. We are continuously developing epigenetic assays for a wide range of immune cells. Combined with our comprehensive immune monitoring solutions, Precision continues to deliver on its promise of “deep science at scale.”